The following protocol is for simultaneous staining of DNA and intracellular Bcl-2 protein It uses a combination of paraformaldehyde and methanol for fixation. Proceed to stained cells and all the yellowtail tetra is performed on. Basic Protocol 6 Surface and Intracellular Staining.

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Wash the cells by adding Flow Cytometry Staining Buffer Use 2 mLtubes or 200 Lwell for microtiter plates Centrifuge at 400-600 x g for 5 minutes at room temperature Discard supernatant.

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